MagBind Plasmid DNA Miniprep Kit KBDM1100
Cat.No：KBDM1100
This kit selectively adsorbs plasmid DNA through a unique combination of embedded magnetic beads and alkaline lysis. It can extract up to 20 μ g of high-purity plasmid DNA from 1-5 mL of overnight cultured bacterial solution, and is suitable for subsequent molecular biology experiments such as PCR amplification, sequencing, in vitro transcription, enzyme digestion reactions, and transformation.
Product Information
Product Name | Cat. No. | Spec. |
MagBind Plasmid DNA Miniprep Kit | KBDM1100-100T | 100T |
Product Description/Introduction
This kit selectively adsorbs plasmid DNA through a unique combination of embedded magnetic beads and alkaline lysis. It can extract up to 20 μg of high-purity plasmid DNA from 1-5 mL of overnight cultured bacterial liquid, suitable for subsequent Molecular biology experiments such as PCR amplification, sequencing, in vitro transcription, enzyme digestion reactions, and transformation.
Storage and Shipping Conditions
RNase A is shipped with wet ice and stored at -20°C. Other reagents are shipped and stored at room temperature, valid for 12 months.
Product Content
Component Number | Component | KBDM1100-100T |
KBDM1100-1 | Solution I | 20 mL (Add RNase A before use) |
KBDM1100-2 | Solution II | 20 mL |
KBDM1100-3 | Neutralization Buffer | 20 mL |
KBDM1100-4 | RNase A | 200 μL |
KBDM1100-5 | SweMag Beads | 3.5 mL |
KBDM1100-6 | Binding Buffer | 20 mL (Add 30 mL anhydrous ethanol before use) |
KBDM1100-7 | Buffer SPW | 24 mL (Add 56 mL anhydrous ethanol before use) |
KBDM1100-8 | Buffer TE | 12 mL |
Manual | 1 copy | |
Before starting (please read carefully)
1. Before use, add all RNase A provided in the kit to Solution I and store at 2-8℃.
2. If there is precipitation in Solution II and Binding Buffer, please heat them at 65℃ until the precipitation disappears, mix well and use (Solution II should not be exposed to air for too long).
3. Add the specified amount of anhydrous ethanol (See bottle) to Binding Buffer and Buffer SPW before use.
4. Bring your own magnetic stand and 1.5 mL sterilized centrifuge tubes.
Assay Protocol/Procedures
1. Take 1-5 mL of fresh bacterial solution cultured overnight, centrifuge at 12,000 rpm for 1 minute, collect in a 1.5 mL centrifuge tube, and discard the supernatant.
2. Add 150 μL of Solution I mixed with RNase A and thoroughly disperse the bacteria using a pipette or Vortex Mixer.
3. Add 150 μL of Solution II and gently invert 8-10 times to fully lyse the bacterial, forming a clear solution (This step should not exceed 5 minutes).
4. Add 150 μL of 4°C pre-cooled Neutralization Buffer, mix well immediately and gently invert 8-10 times (White precipitate appears), and centrifuge at 12,000 rpm for 10 minutes.
5. Transfer carefully the supernatant to a new 1.5 mL sterilized centrifuge tube, add 450 μL of Binding Buffer, invert and mix well, and then add 30 μL of SweMag Beads (SweMag Beads should be vortexed until evenly dispersed before use), gently invert up and down several times until the magnetic beads are evenly dispersed.
6. Leave at room temperature for 5 minutes, during which use a pipette or Vortex Mixer to mix 2-3 times until the magnetic beads are evenly dispersed.
7. Move the centrifuge tube to the magnetic stand and let it stand for 30 seconds until the magnetic beads are completely adsorbed. Gently invert the centrifuge tube several times with the magnetic stand to wash away the residual magnetic beads on the tube wall. After the supernatant is clear, discard it (To avoid affecting the extraction efficiency, do not suck out the magnetic beads).
8. Add 300 μL of Buffer SPW, remove the magnetic stand, and gently blow with a pipette until the magnetic beads are evenly dispersed. Move the centrifuge tube to the magnetic stand and let it stand for 30 seconds, then gently invert the centrifuge tube several times with the magnetic stand to wash away the residual magnetic beads and salt on the tube wall. After the supernatant is clear, discard it (To avoid affecting the extraction efficiency, please make sure to completely remove the residual liquid in the centrifuge tube).
9. Repeat step 8.
10. Open the centrifuge tube cover and let it stand at room temperature for 5-10 minutes or 65°C for 3-5 minutes to completely evaporate ethanol (To avoid excessive drying of magnetic beads and affecting nucleic acid yield).
11. Remove the magnetic stand, add 50-80 μL of Buffer TE or Nuclease-free Water, gently pipette until the magnetic beads are evenly dispersed, and let it stand at room temperature for 3 minutes.
12. Place the centrifuge tube on a magnetic stand until the magnetic beads are completely adsorbed, and transfer the supernatant to a new centrifuge tube to obtain high-purity plasmid DNA.
Note
1. Please read the Product Manual carefully before use.
2. Magnetic beads suspension should avoid freezing during storage.
3. Magnetic beads are prone to precipitation and should be shaken or vortexed before use.
4. Before adding the magnetic beads, other reagents need to be mixed well.
5. Ethanol should be completely evaporated before plasmid DNA elution due to residual ethanol affecting downstream experiments.
6. Do not dry the magnetic beads for long periods of time as this may affect the elution efficiency of plasmid DNA.
7. For your safety and health, please wear lab coats and disposable gloves when operating.
For Research Use Only!
Ver. No.: V1.1-20251028
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