Human Uroplakin 3A (UPK3A) ELISA Kit KB-E0022H

Regulatory Status:RUO

Alternative:UPIII; UPK3

Regulatory Status:

RUO

Alternative:

UPIII; UPK3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.157ng/mL

Specificity:

This kit recognizes Human UPK3A in samples. No significant cross-reactivity or interference between Human UPK3A and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates and other biological fluids

Research Area:

Signal Transduction


| UPK3A


Uroplakin-3a (UP3a) is a protein that is encoded by the UPK3A gene.UP3a is found in the inner membrane of the urinary bladder and contributes to the strength of that membrane and to the membrane's ability to stretch when the bladder is full. It is also found in the renal pelvis, ureter, and prostatic urethra.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)201052.51.250.6250.3120
OD2.41.771.110.70.450.30.20.07
Corrected OD2.331.71.040.630.380.230.13-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human UPK3A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10195
Plasma(n=8)92-105102
Tissue Homogenates(n=8)98-108105






 

| Linearity


Samples were spiked with high concentrations of Human UPK3A and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Serum(n=4)Plasma(n=4)Tissue Homogenates(n=4)
1 : 2Range (%)84-9588-9690-110
Average (%)919496
1 : 4Range (%)89-10388-108105-115
Average (%)9498108











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