Product Name:

Human Interferon Gamma (IFN-γ) ELISA Kit

Cat.#:

KB12152

Regulatory Status:

RUO

Alternative:

IFN-G; IFG; IFI; INFr; IFN, Immune Interferon

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.39-25pg/mL

Sensitivity:

0.15pg/mL

Specificity:

This kit recognizes Human IFN-γ in samples. No significant cross-reactivity or interference between Human IFN-γ and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

| IFN-γ

Interferon gamma (IFNG or IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons.The existence of this interferon, which early in its history was known as immune interferon, was described by E. F. Wheelock as a product of human leukocytes stimulated with phytohemagglutinin, and by others as a product of antigen-stimulated lymphocytes.The IFNG protein is encoded by the IFNG gene.Through cell signaling, interferon gamma plays a role in regulating the immune response of its target cell.A key signaling pathway that is activated by type II IFN is the JAK-STAT signaling pathway.IFNG plays an important role in both innate and adaptive immunity.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human IFN-γ and diluted with Universal Diluent to produce samples with values within the range of the assay.