Product Name:

Human Stromal Cell Derived Factor 1 (SDF-1a;CXCL12) ELISA Kit

Cat.#:

KB34680

Regulatory Status:

RUO

Alternative:

SDF-1a;CXCL12

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.077ng/mL

Specificity:

This kit recognizes Human SDF-1a;CXCL12 in samples. No significant cross-reactivity or interference between Human SDF-1a;CXCL12 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| SDF-1a;CXCL12

This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDF-1a;CXCL12. Add Sample, Standard, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Human SDF-1a;CXCL12 in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Human SDF-1a;CXCL12 in the samples by comparing the OD of the samples to the standard curve.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SDF-1a;CXCL12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human SDF-1a;CXCL12 and diluted with Universal Diluent to produce samples with values within the range of the assay.