Mouse Hepatoma Derived Growth Factor (HDGF) ELISA Kit KB-E13391M

Cat.#:KB-E13391M
Regulatory Status:RUO
Alternative:HMG1L2; High-Mobility Group Protein 1-Like 2

Alternative:

HMG1L2; High-Mobility Group Protein 1-Like 2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

6.25-400pg/mL

Sensitivity:

1.27pg/mL

Specificity:

This kit recognizes Mouse HDGF in samples. No significant cross-reactivity or interference between Mouse HDGF and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer


| HDGF


Hepatoma-derived growth factor (HDGF) also known as high mobility group protein 1-like 2 (HMG-1L2) is a protein that is encoded by the HDGF gene.It is an acidic heparin-binding protein purified from the culture fluid of human hepatocellular carcinoma cell line HuH-7, which is the first member of this protein family to be discovered, and has the function of promoting cell proliferation, differentiation, tissue . It has the functions of promoting cell proliferation and differentiation, organ growth and development, and tissue repair after injury.


| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


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| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Mouse HDGF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


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| Linearity


Samples were spiked with high concentrations of Mouse HDGF and diluted with Universal Diluent to produce samples with values within the range of the assay.


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