Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit KB-E2587H

Regulatory Status:RUO

Alternative:CD107-B; CD107b; LAMP-2C; LAMPB; LGP96; Lysosome-associated membrane glycoprotein 2; CD107 antigen-like family member B

| LAMP2

Lysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3. Its protein, LAMP2, is one of the lysosome-associated membrane glycoProteins.The protein encoded by this gene is a member of a family of membrane glycoProteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of the gene produces three variants - LAMP-2A, LAMP-2B and LAMP-2C.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (pg/mL)40002000100050025012562.50
OD2.281.631.040.690.450.30.220.09
Corrected OD2.191.540.950.60.360.210.13-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human LAMP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10196
Plasma(n=8)92-105102






 

| Linearity


Samples were spiked with high concentrations of Human LAMP2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Serum(n=4)Plasma(n=4)
1 : 2Range (%)84-9588-96
Average (%)9193
1 : 4Range (%)89-10387-108
Average (%)9497








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