Mouse Syntenin 2 (ST2) ELISA Kit KB-E1987M

Regulatory Status:RUO

Alternative:SDCBP2; SITAC18; Syndecan Binding Protein 2


| ST2


Syntenin-2 is a protein that is encoded by the SDCBP2 gene.The protein encoded by this gene contains two class II PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane Proteins, and PDZ-containing Proteins mediate cell signaling and the organization of protein complexes. The encoded protein binds to phosphatidylinositol 4, 5-bisphosphate (PIP2) and plays a role in nuclear PIP2 organization and cell division. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)1052.51.250.6250.3120.1560
OD2.61.741.070.660.430.310.230.09
Corrected OD2.511.650.980.570.340.220.14-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Mouse ST2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10196
Plasma(n=8)89-105102
Tissue Homogenates(n=8)96-108106







 

| Linearity


Samples were spiked with high concentrations of Mouse ST2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Serum(n=4)Plasma(n=4)Tissue Homogenates(n=4)
1 : 2Range (%)86-9588-9690-110
Average (%)919396
1 : 4Range (%)89-10387-108105-115
Average (%)94101108









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