Mouse Bcl2 Associated X Protein (Bax) ELISA Kit KB-E2097R

Regulatory Status:

RUO

Alternative:

BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.14ng/mL

Specificity:

This kit recognizes Mouse Bax in samples. No significant cross-reactivity or interference between Mouse Bax and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

Bax

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that is encoded by the BAX gene.BAX is a member of the Bcl-2 gene family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse Bax spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse Bax and diluted with Universal Diluent to produce samples with values within the range of the assay.