Human Transcription initiation factor TFIID subunit 13 (TAF13) ELISA Kit KB-E2184H

Regulatory Status:

RUO

Alternative:

TAF2K; TAFII18; TATA Box Binding Protein Associated Factor 13; Transcription initiation factor TFIID 18 kDa subunit

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human TAF13 in samples. No significant cross-reactivity or interference between Human TAF13 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids.

Research Area:

Signal Transduction

| TAF13

Transcription initiation factor TFIID subunit 13 is a protein that is encoded by the TAF13 gene.Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved Proteins known as TBP-associated factors or TAFs.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TAF13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human TAF13 and diluted with Universal Diluent to produce samples with values within the range of the assay.