Mouse Olfactomedin 4 (OLFM4) ELISA Kit KB-E12948M

Regulatory Status:

RUO

Alternative:

OLM4; hGC1; hOLfD; OlfD; GW112; GC1; G-CSF-stimulated clone 1 protein; Antiapoptotic protein GW112

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

62.5-4000pg/mL

Sensitivity:

31pg/mL

Specificity:

This kit recognizes Mouse OLFM4 in samples. No significant cross-reactivity or interference between Mouse OLFM4 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids.

Research Area:

Cancer

OLFM4

Olfactomedin 4 is a protein that is encoded by the OLFM4 gene.This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflamed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion.

 Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery 

The recovery of Mouse OLFM4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Mouse OLFM4 and diluted with Universal Diluent to produce samples with values within the range of the assay.