Human Permeability Glycoprotein (P-gp) ELISA Kit KB-E1403H

Regulatory Status:

RUO

Alternative:

CD243; ABCB1; ABC20; CLCS; GP170; MDR1; PGY1; P-Glycoprotein; Multidrug resistance protein 1; ATP-Binding Cassette,Sub-Family B(MDR/TAP),Member 1; Colchicin Sensitivity

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.11ng/mL

Specificity:

This kit recognizes Human P-gp in samples. No significant cross-reactivity or interference between Human P-gp and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| P-gp

P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi, and bacteria, and it likely evolved as a defense mechanism against harmful substances.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human P-gp spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human P-gp and diluted with Universal Diluent to produce samples with values within the range of the assay.