Regulatory Status:RUO
Alternative:CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7
Regulatory Status:
Alternative:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.15-10ng/mL
Sensitivity:
0.06ng/mL
Specificity:
This kit recognizes Mouse CASP7 in samples. No significant cross-reactivity or interference between Mouse CASP7 and analogues was observed.
Sample Type:
Tissue homogenates and other biological fluids
Research Area:
Signal Transduction
Caspase-7, apoptosis-related cysteine peptidase, also known as CASP7, is protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts.Caspase-7 is a member of the caspase (cysteine aspartate protease) family of Proteins, and has been shown to be an executioner protein of apoptosis.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.62 | 0.31 | 0.15 | 0 |
OD | 2.29 | 1.29 | 0.71 | 0.43 | 0.29 | 0.2 | 0.18 | 0.07 |
Corrected OD | 2.22 | 1.22 | 0.64 | 0.36 | 0.22 | 0.13 | 0.11 | - |
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
The recovery of Mouse CASP7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 84-101 | 96 |
Samples were spiked with high concentrations of Mouse CASP7 and diluted with Universal Diluent to produce samples with values within the range of the assay.
Tissue Homogenates(n=4) | ||
1 : 2 | Range (%) | 84-95 |
Average (%) | 91 | |
1 : 4 | Range (%) | 89-103 |
Average (%) | 94 |
CATALOGUE