Human Ras-related protein Rab-5A (RAB5a) ELISA Kit KB-E0255H

Regulatory Status:

RUO

Alternative:

RAB5;RAB5A, Member RAS Oncogene Family

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human RAB5a in samples. No significant cross-reactivity or interference between Human RAB5a and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Neuroscience

| RAB5a

Ras-related protein Rab-5A is a protein that is encoded by the RAB5A gene.RAB5A localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to early endosomes. It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases (V-ATPases) from trans-Golgi network to endocytic vesicles.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human RAB5a spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human RAB5a and diluted with Universal Diluent to produce samples with values within the range of the assay.