Human Kisspeptin 1 (KISS1) ELISA Kit KB-E3495H

Alternative:

Metastin; KiSS-1 Metastasis-Suppressor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

12.97pg/mL

Specificity:

This kit recognizes Human KISS1 in samples. No significant cross-reactivity or interference between Human KISS1 and analogues was observed.

Sample Type:

serum, plasma and other biological fluids

Research Area:

Cancer

| KISS1

Kisspeptins (including kisspeptin-54 (KP-54), formerly known as metastin) are Proteins encoded by the KISS1 gene. Kisspeptins are ligands of the G-protein coupled receptor, GPR54. Kiss1 was originally identified as a human metastasis suppressor gene that has the ability to suppress melanoma and breast cancer metastasis. Kisspeptin-GPR54 signaling has an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty, the extent of which is an area of ongoing research.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human KISS1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human KISS1 and diluted with Universal Diluent to produce samples with values within the range of the assay.