Regulatory Status:RUO
Alternative:RAB7; RAB7A, Member RAS Oncogene Family
Regulatory Status:
Alternative:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.152ng/mL
Specificity:
This kit recognizes Human RAB7A in samples. No significant cross-reactivity or interference between Human RAB7A and analogues was observed.
Sample Type:
tissue homogenates, cell lysates and other biological fluids
Research Area:
Cancer
Ras-related protein Rab-7a is a protein that is encoded by the RAB7A gene.Ras-related protein Rab-7a is involved in endocytosis, which is a process that brings substances into a cell. The process of endocytosis works by folding the cell membrane around a substance outside of the cell (for example a protein) and then forms a vesicle. The vesicle is then brought into the cell and cleaved from the cell membrane. RAB7A plays an important role in the movement of vesicles into the cell as well as with vesicle trafficking.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
Concentration (ng/mL) | 20 | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0 |
OD | 2.36 | 1.36 | 0.8 | 0.48 | 0.34 | 0.28 | 0.23 | 0.09 |
Corrected OD | 2.27 | 1.27 | 0.71 | 0.39 | 0.25 | 0.19 | 0.14 | - |
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
The recovery of Human RAB7A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 89-105 | 102 |
Cell Lysates(n=8) | 96-108 | 103 |
Samples were spiked with high concentrations of Human RAB7A and diluted with Universal Diluent to produce samples with values within the range of the assay.
Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
1 : 2 | Range (%) | 86-96 | 90-110 |
Average (%) | 93 | 96 | |
1 : 4 | Range (%) | 87-108 | 106-115 |
Average (%) | 98 | 110 |
CATALOGUE