Human Ras-related protein Rab-7a (RAB7A) ELISA Kit KB-E3232H

Regulatory Status:RUO
Alternative:RAB7; RAB7A, Member RAS Oncogene Family

Regulatory Status:

RUO

Alternative:

RAB7; RAB7A, Member RAS Oncogene Family

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.152ng/mL

Specificity:

This kit recognizes Human RAB7A in samples. No significant cross-reactivity or interference between Human RAB7A and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Cancer

| RAB7A

Ras-related protein Rab-7a is a protein that is encoded by the RAB7A gene.Ras-related protein Rab-7a is involved in endocytosis, which is a process that brings substances into a cell. The process of endocytosis works by folding the cell membrane around a substance outside of the cell (for example a protein) and then forms a vesicle. The vesicle is then brought into the cell and cleaved from the cell membrane. RAB7A plays an important role in the movement of vesicles into the cell as well as with vesicle trafficking.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

Concentration (ng/mL)201052.51.250.6250.3120
OD2.361.360.80.480.340.280.230.09
Corrected OD2.271.270.710.390.250.190.14-

 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human RAB7A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)89-105102
Cell Lysates(n=8)96-108103






 

| Linearity

Samples were spiked with high concentrations of Human RAB7A and diluted with Universal Diluent to produce samples with values within the range of the assay.

 



Tissue Homogenates(n=4)Cell Lysates(n=4)
1 : 2Range (%)86-9690-110
Average (%)9396
1 : 4Range (%)87-108106-115
Average (%)98110






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