Human Ras-related protein Rab-1A (Rab1A) ELISA Kit KB-E0855H

Regulatory Status:

RUO

Alternative:

RAB1; YPT1; RAB1A, Member RAS Oncogene Family; YPT1-related protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.073ng/mL

Specificity:

This kit recognizes Human Rab1A in samples. No significant cross-reactivity or interference between Human Rab1A and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids.

Research Area:

Signal Transduction

| Rab1A

Ras-related protein Rab-1A is a protein that is encoded by the RAB1A gene.This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. Diseases associated with RAB1A include Choroideremia and Esophageal Neuroendocrine Tumor. Among its related pathways are Transport to the Golgi and subsequent modification and Cell Cycle, Mitotic.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Rab1A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human Rab1A and diluted with Universal Diluent to produce samples with values within the range of the assay.