Human Ribonucleoside-diphosphate reductase large subunit (RRM1) ELISA Kit KB-E0549H

Regulatory Status:

RUO

Alternative:

R1; RIR1; RR1; Ribonucleotide Reductase M1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human RRM1 in samples. No significant cross-reactivity or interference between Human RRM1 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Metabolism

| RRM1

Ribonucleoside-diphosphate reductase large subunit is an enzyme that is encoded by the RRM1 gene.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human RRM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human RRM1 and diluted with Universal Diluent to produce samples with values within the range of the assay.