Human Alpha-2-Macroglobulin (α2-MG) ELISA Kit KB-E2387H

Regulatory Status:

RUO

Alternative:

A2MG; A2mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

3.90-250ng/mL

Sensitivity:

1.37ng/mL

Specificity:

This kit recognizes Human α2-MG in samples. No significant cross-reactivity or interference between Human α2-MG and analogues was observed.

Sample Type:

Serum, plasma, urine and other biological fluids

Research Area:

Cancer

| α2-MG

α2-Macroglobulin (α2M) or alpha-2-macroglobulin is a large (720 KDa) plasma protein found in the blood. It is mainly produced by the liver, and also locally synthesized by macrophages, fibroblasts, and adrenocortical cells. It is encoded by the A2M gene.α2-Macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. α2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-β, insulin, and IL-1β.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human α2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human α2-MG and diluted with Universal Diluent to produce samples with values within the range of the assay.