Mouse Cluster Of Differentiation 19 (CD19) ELISA Kit KB-E12867M

Regulatory Status:

RUO

Alternative:

B4; B-lymphocyte surface antigen B4; T-cell surface antigen Leu-12

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Mouse CD19 in samples. No significant cross-reactivity or interference between Mouse CD19 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| CD19

B-lymphocyte antigen CD19, also known as CD19 molecule (Cluster of Differentiation 19), B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12 and CVID3 is a transmembrane protein that is encoded by the gene CD19.In humans, CD19 is expressed in all B lineage cells.Contrary to some early doubts, human plasma cells do express CD19,as confirmed by others.CD19 plays two major roles in human B cells: on the one hand, it acts as an adaptor protein to recruit cytoplasmic signaling Proteins to the membrane; on the other, it works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse CD19 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse CD19 and diluted with Universal Diluent to produce samples with values within the range of the assay.