Human Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) ELISA Kit KB-E0934H

Regulatory Status:

RUO

Alternative:

Leucine Rich Alpha-2-Glycoprotein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

6.25-400ng/mL

Sensitivity:

2.95ng/mL

Specificity:

This kit recognizes Human LRG1 in samples. No significant cross-reactivity or interference between Human LRG1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

| LRG1

Leucine-rich alpha-2-glycoprotein 1 is a protein which is encoded by the gene LRG1.The leucine-rich repeat (LRR) family of Proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation.LRG1 has been shown to be involved in promoting neovascularization (new blood vessel growth) through causing a switch in transforming growth factor beta (TGFbeta) signaling in endothelial cells. LRG1 binds to the accessory receptor endoglin and promotes signaling via the ALK1-Smad1/5/8 pathway.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human LRG1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human LRG1 and diluted with Universal Diluent to produce samples with values within the range of the assay.