Human Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) ELISA Kit KB-E0356H

Regulatory Status:

RUO

Alternative:

SPPase1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.081ng/mL

Specificity:

This kit recognizes Human SGPP1 in samples. No significant cross-reactivity or interference between Human SGPP1 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

SGPP1

Sphingosine-1-phosphate phosphatase 1 is an enzyme that is encoded by the SGPP1 gene.Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SGPP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human SGPP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.