Human WNT1 Inducible Signaling Pathway Protein 1 (WISP1) ELISA Kit KB-E0544H

Regulatory Status:RUO
Alternative:CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein

Regulatory Status:

RUO

Alternative:

CCN4; WISP1c; WISP1i; WISP1tc; CCN family member 4; Wnt-1-induced secreted protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

11.97pg/mL

Specificity:

This kit recognizes Human WISP1 in samples. No significant cross-reactivity or interference between Human WISP1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Research Area:

Signal Transduction


| WISP1

WNT1-inducible-signaling pathway protein 1 (WISP-1), is a member of the CCN protein family and should correctly be referred to as CCN4 as suggested by the International CCN Society. It is a matricellular protein that is encoded by the WISP1 gene.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

Concentration (pg/mL)2000100050025012562.531.250
OD2.321.931.310.850.510.390.280.1
Corrected OD2.221.831.210.750.410.290.18-

 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human WISP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10196
Plasma(n=8)92-105102






 

| Linearity

Samples were spiked with high concentrations of Human WISP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 



Serum(n=4)Plasma(n=4)
1 : 2Range (%)84-9588-96
Average (%)9193
1 : 4Range (%)89-10387-108
Average (%)9598







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