Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit KB-E0644H

Regulatory Status:

RUO

Alternative:

ISGF-3; STAT91; Transcription Factor ISGF-3 Components p91/p84

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.11ng/mL

Specificity:

This kit recognizes Human STAT1 in samples. No significant cross-reactivity or interference between Human STAT1 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| STAT1

Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. It is a member of the STAT protein family.All STAT molecules are phosphorylated by receptor associated kinases, that causes activation, dimerization by forming homo- or heterodimers and finally translocate to nucleus to work as transcription factors. Specifically STAT1 can be activated by several ligands such as Interferon alpha (IFNα), Interferon gamma (IFNγ), Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Interleukin 6 (IL-6), or IL-27.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human STAT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human STAT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.