Human Protein Wnt-9b (WNT9B) ELISA Kit KB-E0407H

Regulatory Status:

RUO

Alternative:

WNT15; WNT14B;Wingless Type MMTV Integration Site Family, Member 9B

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.076ng/mL

Specificity:

This kit recognizes Human WNT9B in samples. No significant cross-reactivity or interference between Human WNT9B and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| WNT9B

Protein Wnt-9b (formerly Wnt15) is a protein that is encoded by the WNT9B gene.The Wnt family of genes produce glycolipoProteins that are involved with signaling and developmental processes. Like other Wnt genes, Wnt-9b codes for the Wnt-9b protein which participates in the canonical Wnt/β-catenin signaling pathway. Wnt-9b is a gene found on chromosome 17 in region 17q21. It can be traced to function in the establishment of the kidneys, because Wnt9 is critical for morphogenesis of the nephron. This gene can impact kidney function in more than one way.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human WNT9B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human WNT9B and diluted with Universal Diluent to produce samples with values within the range of the assay.