Mouse Dickkopf Related Protein 1 (DKK1) ELISA Kit KB-E11463M

Regulatory Status:

RUO

Alternative:

SK; Dickkopf Homolog 1(Xenopus Laevis)

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.42ng/mL

Specificity:

This kit recognizes Mouse DKK1 in samples. No significant cross-reactivity or interference between Mouse DKK1 and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids.

Research Area:

Signal Transduction

DKK1

Dickkopf-related protein 1 is a protein that is encoded by the DKK1 gene.This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm.The dickkopf protein encoded by DKK1 is an antagonist of the Wnt/β-catenin signalling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway.This inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse DKK1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse DKK1 and diluted with Universal Diluent to produce samples with values within the range of the assay.