Human Connexin 40 (CX40) ELISA Kit KB-E1606H

Regulatory Status:

RUO

Alternative:

GJA5; GJ-A5; Gap Junction Protein Alpha 5

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.14ng/mL

Specificity:

This kit recognizes Human CX40 in samples. No significant cross-reactivity or interference between Human CX40 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

| CX40

Gap junction alpha-5 protein (GJA5), also known as connexin 40 (Cx40) — is a protein that in humans is encoded by the GJA5 gene.This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.

 | Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human CX40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human CX40 and diluted with Universal Diluent to produce samples with values within the range of the assay.