Human Exosome Component 7 (EXOSC7) ELISA Kit KB-E1609H

Regulatory Status:RUO

Alternative:Rrp42p; RRP42; EAP1; p8; hRrp42p; Ribosomal RNA-processing protein 42; Exosome complex component RRP42

Regulatory Status:

RUO

Alternative:

Rrp42p; RRP42; EAP1; p8; hRrp42p; Ribosomal RNA-processing protein 42; Exosome complex component RRP42

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.072ng/mL

Specificity:

This kit recognizes Human EXOSC7 in samples. No significant cross-reactivity or interference between Human EXOSC7 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction


| EXOSC7


Exosome component 7, also known as EXOSC7, is a gene, the protein product of which is part of the exosome complex.Predicted to enable mRNA 3'-UTR AU-rich region binding activity. Involved in RNA catabolic process and RNA processing. Part of cytosol; exosome (RNase complex); and nucleolus.EXOSC7 (Exosome Component 7) is a Protein Coding gene. Diseases associated with EXOSC7 include Pontocerebellar Hypoplasia, Type 1C and Pontocerebellar Hypoplasia, Type 1B.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)1052.51.250.6250.3120.1560
OD2.231.420.830.50.310.230.180.08
Corrected OD2.151.340.750.420.230.150.1-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human EXOSC7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)83-10196
Cell Lysates(n=8)92-105103






 

| Linearity


Samples were spiked with high concentrations of Human EXOSC7 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)Cell Lysates(n=4)
1 : 2Range (%)82-9588-96
Average (%)9192
1 : 4Range (%)89-10387-108
Average (%)94100








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