Mouse Gremlin 1 (GREM1) ELISA Kit KB-E14009M

Regulatory Status:

RUO

Alternative:

PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.25-2000pg/mL

Sensitivity:

14.73pg/mL

Specificity:

This kit recognizes Mouse GREM1 in samples. No significant cross-reactivity or interference between Mouse GREM1 and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids

Research Area:

Signal Transduction

| GREM1

Gremlin is an inhibitor in the TGF beta signaling pathway. It primarily inhibits bone morphogenesis and is implicated in disorders of increased bone formation and several cancers.Gremlin1, previously known as Drm, is a highly conserved 20.7-kDa, 184 amino acid glycoprotein part of the DAN family and is a cysteine knot-secreted protein.Gremlin1 was first identified in differential screening as a transcriptional down-regulated gene in v-mos-transformed rat embryonic fibroblasts.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse GREM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse GREM1 and diluted with Universal Diluent to produce samples with values within the range of the assay.