Mouse Fibronectin (FN) ELISA Kit KB-E12099M

Regulatory Status:

RUO

Alternative:

FN1; CIG; FINC; LETS; MSF; GFND2; Anastellin; Migration-Stimulating Factor; Cold-Insoluble Globulin; Large, External, Transformation-Sensitive Protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.54ng/mL

Specificity:

This kit recognizes Mouse FN in samples. No significant cross-reactivity or interference between Mouse FN and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

FN

Fibronectin is a high-molecular weight (~500-~600 kDa)[5] glycoprotein of the extracellular matrix that binds to membrane-spanning receptor Proteins called integrins. Fibronectin also binds to other extracellular matrix Proteins such as collagen, fibrin, and heparan sulfate proteoglycans.Fibronectin exists as a protein dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds.The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse FN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Mouse FN and diluted with Universal Diluent to produce samples with values within the range of the assay.