Human Mothers Against Decapentaplegic Homolog 4 (Smad4) ELISA Kit KB-E1713H

Regulatory Status:

RUO

Alternative:

DPC4; JIP; MADH4; Deletion target in pancreatic carcinoma 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.11ng/mL

Specificity:

This kit recognizes Human Smad4 in samples. No significant cross-reactivity or interference between Human Smad4 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| Smad4

SMAD4, also called SMAD family member 4, Mothers against decapentaplegic homolog 4, or DPC4 (Deleted in Pancreatic Cancer-4) is a highly conserved protein present in all metazoans. It belongs to the SMAD family of transcription factor Proteins, which act as mediators of TGF-β signal transduction. The TGFβ family of cytokines regulates critical processes during the lifecycle of metazoans, with important roles during embryo development, tissue homeostasis, regeneration, and immune regulation.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Smad4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human Smad4 and diluted with Universal Diluent to produce samples with values within the range of the assay.