Rat Matrix Metalloproteinase 3 (MMP-3) ELISA Kit

Alternative:

SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Rat MMP-3 in samples. No significant cross-reactivity or interference between Rat MMP-3 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Research Area:

Cancer

MMP-3

Stromelysin-1 also known as matrix metalloproteinase-3 (MMP-3) is an enzyme that is encoded by the MMP3 gene. The MMP3 gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. MMP-3 has an estimated molecular weight of 54 kDa.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix Proteins and during tissue remodeling in normal physiological processes, such as embryonic development and reproduction, as well as in disease processes, such as arthritis, and tumour metastasis. Most MMPs are secreted as inactive proProteins which are activated when cleaved by extracellular proteinases.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery

The recovery of Rat MMP-3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 Linearity

Samples were spiked with high concentrations of Rat MMP-3 and diluted with Universal Diluent to produce samples with values within the range of the assay.