Mouse Matrix Metalloproteinase 13 (MMP-13) ELISA Kit KB-E12420M

Alternative:

CLG3; Collagenase 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.2-2000pg/mL

Sensitivity:

14.5pg/mL

Specificity:

This kit recognizes Mouse MMP-13 in samples. No significant cross-reactivity or interference between Mouse MMP-13 and analogues was observed.

Sample Type:

serum, plasma and other biological fluids

Research Area:

Cancer

| MMP-13

Collagenase 3 is an enzyme that is encoded by the MMP13 gene. It is a member of the matrix metalloproteinase (MMP) family. Like most MMPs, it is secreted as an inactive pro-form. MMP-13 has a predicted molecular weight around 54 kDa.It is activated once the pro-domain is cleaved, leaving an active enzyme composed of the catalytic domain and the hemopexin-like domain PDB: 1PEX. Although the actual mechanism has not been described, the hemopexin domain participates in collagen degradation, the catalytic domain alone being particularly inefficient in collagen degradation. During embryonic development, MMP-13 is expressed in the skeleton as required for restructuring the collagen matrix for bone mineralization.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse MMP-13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse MMP-13 and diluted with Universal Diluent to produce samples with values within the range of the assay.