Mouse Matrix Metalloproteinase 14 (MMP-14) ELISA Kit KB-E12579M

| Linearity

Samples were spiked with high concentrations of Mouse MMP-14 and diluted with Universal Diluent to produce samples with values within the range of the assay.

Alternative:

MMP-X1; MT1-MMP; MTMMP1; Membrane Inserted; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.56ng/mL

Specificity:

This kit recognizes Mouse MMP-14 in samples. No significant cross-reactivity or interference between Mouse MMP-14 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| MMP-14

Matrix metalloproteinase-14 is an enzyme that is encoded by the MMP14 gene.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Deficits in MMP14 leads to premature aging, short lifespan, and cell senescence in mice, suggesting an important role of MMP14 in extracellular matrix remodeling during aging. Most MMP's are secreted as inactive pro-Proteins which are activated when cleaved by extracellular proteinases.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse MMP-14 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse MMP-14 and diluted with Universal Diluent to produce samples with values within the range of the assay.