Mouse Matrix Metalloproteinase 2 (MMP-2) ELISA Kit KB-E13758M

Regulatory Status:

RUO

Alternative:

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.76ng/mL

Specificity:

This kit recognizes Mouse MMP-2 in samples. No significant cross-reactivity or interference between Mouse MMP-2 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates and other biological fluids

Research Area:

Cancer

| MMP-2

72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that is encoded by the MMP2 gene.he MMP2 gene is located on chromosome 16 at position 12.2.Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proProteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse MMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse MMP-2 and diluted with Universal Diluent to produce samples with values within the range of the assay.