Human Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22) ELISA Kit KB-E0848H

Regulatory Status:RUO

Alternative:TSC22D1; TGFB1I4; TSC22; hucep-2; Cerebral protein 2; Transforming Growth Factor Beta 1 Induced Transcript 4; TSC22 Domain Family,Member 1

Regulatory Status:

RUO

Alternative:

TSC22D1; TGFB1I4; TSC22; hucep-2; Cerebral protein 2; Transforming Growth Factor Beta 1 Induced Transcript 4; TSC22 Domain Family,Member 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human TSC22 in samples. No significant cross-reactivity or interference between Human TSC22 and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids

Research Area:

Signal Transduction


| TSC22


TSC22 domain family protein 1 is a protein that is encoded by the TSC22D1 gene.TSC22 encodes a transcription factor and belongs to the large family of early response genes.TSC22D1 forms homodimers via its conserved leucine zipper domain and heterodimerizes with TSC22D4. TSC22D1 has transcriptional repressor activity.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)201052.51.250.620.310
OD2.041.490.970.630.410.290.240.09
Corrected OD1.951.40.880.540.320.20.15-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human TSC22 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)92-105102






 

| Linearity


Samples were spiked with high concentrations of Human TSC22 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)
1 : 2Range (%)88-96
Average (%)93
1 : 4Range (%)87-108
Average (%)98







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