Human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ELISA Kit KB-E0324H

Regulatory Status:RUO

Alternative:C14orf141; LTBP3

Regulatory Status:

RUO

Alternative:

C14orf141; LTBP3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human LTBP2 in samples. No significant cross-reactivity or interference between Human LTBP2 and analogues was observed.

Sample Type:

Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction


| LTBP2


Latent-transforming growth factor beta-binding protein 2 is a protein that is encoded by the LTBP2 gene.The protein encoded by this gene belongs to the family of latent transforming growth factor (TGF)-beta binding Proteins (LTBP), which are extracellular matrix Proteins with multi-domain structure. This protein is the largest member of the LTBP family possessing unique regions and with most similarity to the fibrillins.It has thus been suggested that it may have multiple functions: as a member of the TGF-beta latent complex, as a structural component of microfibrils, and a role in cell adhesion.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)201052.51.250.620.310
OD2.181.571.030.620.420.320.260.1
Corrected OD2.081.470.930.520.320.220.16-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human LTBP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)84-10196
Plasma(n=8)92-105102
Cell Culture Supernates(n=8)97-108105






 

| Linearity


Samples were spiked with high concentrations of Human LTBP2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Serum(n=4)Plasma(n=4)Cell Culture Supernates(n=4)
1 : 2Range (%)84-9588-9690-110
Average (%)919396
1 : 4Range (%)89-10387-108105-115
Average (%)9498108










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