Human Stanniocalcin 2 (STC-2) ELISA Kit KB-E1047H

Regulatory Status:RUO

Alternative:STCRP; Stanniocalcin-related protein

Regulatory Status:

RUO

Alternative:

STCRP; Stanniocalcin-related protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.2-2000pg/mL

Sensitivity:

15.2pg/mL

Specificity:

This kit recognizes Human STC-2 in samples. No significant cross-reactivity or interference between Human STC-2 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction


    STC-2


    Stanniocalcin-2 is a protein that is encoded by the STC2 gene.This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues.


     

    | Typical data


    As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


      

    Concentration (pg/mL)2000100050025012562.531.250
    OD2.281.791.3710.650.390.260.09
    Corrected OD2.191.71.280.910.560.30.17-

     






    | Repeatability


    Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

     

    | Recovery


    The recovery of Human STC-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


     

    Sample TypeRange(%)Average Recovery(%)
    Serum(n=8)84-10296
    Plasma(n=8)92-105103
    Cell Culture Supernates(n=8)96-108105







     

    | Linearity


    Samples were spiked with high concentrations of Human STC-2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


     



    Serum(n=4)Plasma(n=4)Cell Culture Supernates(n=4)
    1 : 2Range (%)84-9585-9690-110
    Average (%)919396
    1 : 4Range (%)89-10387-108105-115
    Average (%)9498110











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