Human Cysteine Rich Secretory Protein 2 (CRISP2) ELISA Kit KB-E3647H

Regulatory Status:RUO

Alternative:TSP1; CRISP-2; GAPDL5; TPX1; CT36; Testis Specific Protein 1; Cancer/Testis Antigen 36; Testis-specific protein TPX-1

Regulatory Status:

RUO

Alternative:

TSP1; CRISP-2; GAPDL5; TPX1; CT36; Testis Specific Protein 1; Cancer/Testis Antigen 36; Testis-specific protein TPX-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human CRISP2 in samples. No significant cross-reactivity or interference between Human CRISP2 and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids

Research Area:

Signal Transduction


| CRISP2


Cysteine-rich secretory protein 2 is a cysteine-rich secretory protein that is encoded by the CRISP2 gene.Predicted to be located in extracellular region. Predicted to be active in extracellular space. Diseases associated with CRISP2 include Male Infertility. An important paralog of this gene is CRISP3.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)1052.51.250.6250.3120.1560
OD2.171.741.170.780.470.320.230.08
Corrected OD2.091.661.090.70.390.240.15-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human CRISP2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)95-108105






 

| Linearity


Samples were spiked with high concentrations of Human CRISP2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)
1 : 2Range (%)92-110
Average (%)96
1 : 4Range (%)105-115
Average (%)110









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