Human C-Type Lectin Domain Family 11, Member A (CLEC11A) ELISA Kit KB-E2809H

Regulatory Status:RUO

Alternative:SCGF; P47; LSLCL; CLECSF3; Stem Cell Growth Factor; Lymphocyte Secreted C-Type Lectin; C-type lectin superfamily member 3

Regulatory Status:

RUO

Alternative:

SCGF; P47; LSLCL; CLECSF3; Stem Cell Growth Factor; Lymphocyte Secreted C-Type Lectin; C-type lectin superfamily member 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.94-60ng/mL

Sensitivity:

0.5ng/mL

Specificity:

This kit recognizes Human CLEC11A in samples. No significant cross-reactivity or interference between Human CLEC11A and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Research Area:

Signal Transduction


| CLEC11A


C-type lectin domain family 11 member A is a protein that is encoded by the CLEC11A gene.This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)6030157.53.751.880.940
OD2.311.590.990.60.40.270.190.07
Corrected OD2.241.520.920.530.330.20.12-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human CLEC11A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Serum(n=8)83-10196
Plasma(n=8)92-105102
Tissue Homogenates(n=8)96-108103






 

| Linearity


Samples were spiked with high concentrations of Human CLEC11A and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Serum(n=4)Plasma(n=4)Tissue Homogenates(n=4)
1 : 2Range (%)85-9588-9690-110
Average (%)919396
1 : 4Range (%)89-10387-108105-115
Average (%)9498109










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