Human Transcription termination factor 1 (TTF1) ELISA Kit KB-E0417H

Regulatory Status:

RUO

Alternative:

TTF1; NKX2-1; BCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; NK2 Homeobox 1; Benign Chorea; Thyroid nuclear factor 1; Thyroid-specific enhancer-binding protein;thyroid transcription factor 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.083ng/mL

Specificity:

This kit recognizes Human TTF1 in samples. No significant cross-reactivity or interference between Human TTF1 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| TTF1

Transcription termination factor 1 is a protein that is encoded by the TTF1 gene.This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TTF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human TTF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.