Human Transcription Factor 7 Like 2 (TCF7L2) ELISA Kit KB-E0318H

Regulatory Status:RUO

Alternative:TCF4; HMG box transcription factor 4;Transcription Factor 7 Like Protein 2

Regulatory Status:

RUO

Alternative:

TCF4; HMG box transcription factor 4;Transcription Factor 7 Like Protein 2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.077ng/mL

Specificity:

This kit recognizes Human TCF7L2 in samples. No significant cross-reactivity or interference between Human TCF7L2 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction


| TCF7L2


Transcription factor 7-like 2 (T-cell specific, HMG-box), also known as TCF7L2 or TCF4, is a protein acting as a transcription factor that is encoded by the TCF7L2 gene. The TCF7L2 gene is located on chromosome 10q25.2–q25.3, contains 19 exons.As a member of the TCF family, TCF7L2 can form a bipartite transcription factor and influence several biological pathways, including the Wnt signalling pathway.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)1052.51.250.6250.3120.1560
OD2.61.741.070.660.430.310.230.09
Corrected OD2.511.650.980.570.340.220.14-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human TCF7L2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)84-10396
Cell Culture Supernates(n=8)92-105101






 

| Linearity


Samples were spiked with high concentrations of Human TCF7L2 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)Cell Culture Supernates(n=4)
1 : 2Range (%)84-9585-96
Average (%)9193
1 : 4Range (%)89-10387-108
Average (%)9598









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