Human Focal adhesion kinase 1 (FAK) ELISA Kit KB-E2312H

Regulatory Status:

RUO

Alternative:

FAK; FADK; FAK1; Pp125FAK; PPP1R71; Focal Adhesion Kinase; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

1.56-100ng/mL

Sensitivity:

0.8ng/mL

Specificity:

This kit recognizes Human FAK in samples. No significant cross-reactivity or interference between Human FAK and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids.

Research Area:

Signal Transduction

| FAK

PTK2 protein tyrosine kinase 2 (PTK2), also known as focal adhesion kinase (FAK), is a protein that is encoded by the PTK2 gene. PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion (how cells stick to each other and their surroundings) and spreading processes (how cells move around). It has been shown that when FAK was blocked, breast cancer cells became less metastatic due to decreased mobility.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human FAK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human FAK and diluted with Universal Diluent to produce samples with values within the range of the assay.