Human Transformer-2 protein homolog beta (TRA2b) ELISA Kit KB-E0174H

Regulatory Status:

RUO

Alternative:

SRFS10; Htra2-beta; TRA2-BETA; TRA2B; Splicing Factor,Arginine/Serine-Rich 10; Transformer 2 Beta

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.07ng/mL

Specificity:

This kit recognizes Human TRA2b in samples. No significant cross-reactivity or interference between Human TRA2b and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| TRA2b

Transformer-2 protein homolog beta, also known as TRA2B previously known as splicing factor, arginine/serine-rich 10 (transformer 2 homolog, Drosophila) (SFRS10), is a protein that is encoded by the TRA2B gene.TRA2B contains a poison exon whose inclusion is autoregulated by TRA2B protein. Proper regulation of poison exon inclusion in TRA2B is important for T cell fate.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TRA2b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human TRA2b and diluted with Universal Diluent to produce samples with values within the range of the assay.