Human Necdin (NDN) ELISA Kit KB-E3026H

Regulatory Status:

RUO

Alternative:

PWCR; Necdin Homolog

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.075ng/mL

Specificity:

This kit recognizes Human NDN in samples. No significant cross-reactivity or interference between Human NDN and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids.

Research Area:

Neuroscience

| NDN

Necdin is a protein that is encoded by the NDN gene.This intronless gene is located in the Prader-Willi syndrome (PWS) deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mice suggest that the protein encoded by this gene may suppress growth in postmitotic neurons.Necdin is used to stimulate growth regulation and DNA-dependent transcription regulation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human NDN spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human NDN and diluted with Universal Diluent to produce samples with values within the range of the assay.