Human Hedgehog Homolog, Sonic (SHH) ELISA Kit KB-E1568H

Regulatory Status:

RUO

Alternative:

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

31.27pg/mL

Specificity:

This kit recognizes Human SHH in samples. No significant cross-reactivity or interference between Human SHH and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| SHH

Sonic hedgehog protein (SHH) is encoded for by the SHH gene.The protein is named after the video game character Sonic the Hedgehog.This signaling molecule is key in regulating embryonic morphogenesis in all animals. SHH controls organogenesis and the organization of the central nervous system, limbs, digits and many other parts of the body. Sonic hedgehog is a morphogen that patterns the developing embryo using a concentration gradient characterized by the French flag model. This model has a non-uniform distribution of SHH molecules which governs different cell fates according to concentration.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human SHH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human SHH and diluted with Universal Diluent to produce samples with values within the range of the assay.