Mouse R-Spondin 1 (RSPO1) ELISA Kit KB-E11995M

Regulatory Status:

RUO

Alternative:

RSPONDIN; Roof plate-specific spondin-1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Mouse RSPO1 in samples. No significant cross-reactivity or interference between Mouse RSPO1 and analogues was observed.

Sample Type:

tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

| RSPO1

R-spondin-1 is a secreted protein that is encoded by the RSPO1 gene, found on chromosome 1. It interacts with WNT4 in the process of female sex development. Loss of function can cause female to male sex reversal. Furthermore, it promotes canonical WNT/β catenin signaling.The protein has two cysteine-rich, furin-like domains and one thrombospondin type 1 domain.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse RSPO1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse RSPO1 and diluted with Universal Diluent to produce samples with values within the range of the assay.