Rat Nuclear Receptor Coactivator 3 (NCOA3) ELISA Kit KB-E23835R

Regulatory Status:

RUO

Alternative:

RAC3; ACTR; AIB1; CAGH16; CTG26; SRC3; TNRC14; TNRC16; TRAM1; pCIP; Amplified In Breast 1; Steroid Receptor Coactivator-3 ; Thyroid Hormone Receptor Activator Molecule 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.153ng/mL

Specificity:

This kit recognizes Rat NCOA3 in samples. No significant cross-reactivity or interference between Rat NCOA3 and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids.

Research Area:

Immunology

| NCOA3

The nuclear receptor coactivator 3 also known as NCOA3 is a protein that is encoded by the NCOA3 gene.NCOA3 is also frequently called 'amplified in breast 1' (AIB1), steroid receptor coactivator-3 (SRC-3), or thyroid hormone receptor activator molecule 1 (TRAM-1).NCOA3 is a transcriptional coactivator protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCOA3 is recruited to DNA promotion sites by ligand-activated nuclear receptors. NCOA3, in turn, acylates histones, which makes downstream DNA more accessible to transcription. Hence, NCOA3 assists nuclear receptors in the upregulation of gene expression.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat NCOA3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Rat NCOA3 and diluted with Universal Diluent to produce samples with values within the range of the assay.