Human Alpha-Methylacyl Coenzyme A Racemase (aMACR) ELISA Kit KB-E1923H

Regulatory Status:

RUO

Alternative:

RACE; 2-methylacyl-CoA racemase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.074ng/mL

Specificity:

This kit recognizes Human aMACR in samples. No significant cross-reactivity or interference between Human aMACR and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Cell Biology

| aMACR

α-Methylacyl-CoA racemase (AMACR,) is an enzyme that is encoded by the AMACR gene.In mammalian cells, the enzyme is responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. This transformation is required in order to degrade (2R)-methylacyl-CoA esters by β-oxidation, which process requires the (2S)-epimer. The enzyme is known to be localised in peroxisomes and mitochondria, both of which are known to β-oxidize 2-methylacyl-CoA esters.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human aMACR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human aMACR and diluted with Universal Diluent to produce samples with values within the range of the assay.