Regulatory Status:RUO
Alternative:RAB37, Member RAS Oncogene Family
Regulatory Status:
Alternative:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.312-20ng/mL
Sensitivity:
0.16ng/mL
Specificity:
This kit recognizes Human RAB37 in samples. No significant cross-reactivity or interference between Human RAB37 and analogues was observed.
Sample Type:
Tissue homogenates and other biological fluids.
Research Area:
Signal Transduction
Ras-related protein Rab-37 is a protein that is encoded by the RAB37 gene.Rab Proteins are low molecular mass GTPases that are critical regulators of vesicle trafficking. Diseases associated with RAB37 include Esophageal Atresia/Tracheoesophageal Fistula. Among its related pathways are Innate Immune System and Metabolism of Proteins.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
Concentration (ng/mL) | 20 | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0 |
OD | 2.41 | 1.84 | 1.21 | 0.85 | 0.52 | 0.33 | 0.21 | 0.08 |
Corrected OD | 2.33 | 1.76 | 1.13 | 0.77 | 0.44 | 0.25 | 0.13 | - |
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
The recovery of Human RAB37 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 82-102 | 96 |
Samples were spiked with high concentrations of Human RAB37 and diluted with Universal Diluent to produce samples with values within the range of the assay.
Tissue Homogenates(n=4) | ||
1 : 2 | Range (%) | 84-95 |
Average (%) | 91 | |
1 : 4 | Range (%) | 89-103 |
Average (%) | 99 |
CATALOGUE