Rat Ras-related protein Rap-2b (RAP2B) ELISA Kit KB-E23871R

Regulatory Status:

RUO

Alternative:

RAP2B, Member Of RAS Oncogene Family; Ras Family Small GTP Binding Protein RAP2B

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.083ng/mL

Specificity:

This kit recognizes Rat RAP2B in samples. No significant cross-reactivity or interference between Rat RAP2B and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Cancer

| RAP2B

Ras-related protein Rap-2b is a protein that is encoded by the RAP2B gene.RAP2B belongs to the Ras-related protein family.This intronless gene belongs to a family of RAS-related genes. The Proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS Proteins and have numerous structural features in common. The most striking difference between the RAP and RAS Proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP Proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat RAP2B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Rat RAP2B and diluted with Universal Diluent to produce samples with values within the range of the assay.